Metabolically Engineered Organisms For The Production Of Hydrogen And Hydrogenase

The present application relates to the use of metabolically-engineered microbial cells for the production of hydrogen and hydrogenase enzymes. The microbial cells are strains of E. coli which are genetically engineered to optimize the cell for production of hydrogen or active hydrogenase. The strains of E. coli are transformed with at least one expression vector directed towards the biosynthesis of a hydrogenase enzyme. Methods of hydrogen production, fuel-cell systems and recombinant fuel-cell catalysts are also provided.