Directed Evolution Methods For Improving Polypeptide Folding And Solubility And Superfolder Fluorescent Proteins Generated Thereby

The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.