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Nanoplasmonic Molecular Ruler For Nuclease Activity And Dna Footprinting

Nanoplasmonic Molecular Ruler For Nuclease Activity And Dna Footprinting

Description

Abstract:

This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplamonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

Issue/Publication date:

07/03/2014

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