The invention relates to methods of modifying a plastid genome using sequence specific nucleases, as well as reverse transcriptase polypeptides and plastid modification cassettes. The invention further relates to methods of modifying a plastid or a mitochondrial genome using ATP-dependent DNA ligase D (LigD) and DNA-binding protein Ku (Ku). Also included are the plants, plant cells, and seeds produced by these methods.
Issue/Publication date:
06/13/2019
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